Application of Barley Tweaky Spike Mutants for the Study of Effects of Plant Immunity-Related Substances

Barley developmental mutants tweaky spike (tw) with disturbed auxin pathways possess a unique feature of an increased level mouldy germinating grains (MGG), which serves as convenient model to investigate the effects plant immunity-related substances. The 2,4-dichlorophenoxyacetic acid (2,4-D), inhibitors, salicylic (SA), and trans-cinnamic (TCA) were studied using tw-WT system in surface-sterilized unsterilized under high rates natural infection. Significant differences among allelic tw revealed at MGG response 2,4-D, SA, TCA. most effective means against sterilization 2,4-D inhibited root growth tw2 mutants, occurring only not sterilized grains, while opposite was observed for TCA SA. mutations influenced variations seed-borne fungal spectra, decreasing frequency Bipolaris sorokiniana increasing Fusarium spp. Hypochlorite-based surface methods should be used caution studies where action exogenous will analysed grains. Auxin pathway disturbances specific pleiotropic are generally restricted organogenesis but germination events.